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Verma, Digvijay
- Camphora Augments Humoral Mediated Immunity and Decreases Delayed type Hypersensitivity in BALB/c Mice
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Authors
Affiliations
1 Drug Standardization Department, Dr Anjali Chatterji Regional Research Institute for Homeopathy, Under Central Council for Research in Homeopathy, Kolkata - 700035, West Bengal, IN
2 Drug Standardization Department, Central Council for Research in Homeopathy, New Delhi - 110058, IN
3 Drug Standardization Department, Dr D P Rastogi Central Research Institute for Homeopathy, Under Central Council for Research in Homeopathy, Noida - 201301, Uttar Pradesh, IN
1 Drug Standardization Department, Dr Anjali Chatterji Regional Research Institute for Homeopathy, Under Central Council for Research in Homeopathy, Kolkata - 700035, West Bengal, IN
2 Drug Standardization Department, Central Council for Research in Homeopathy, New Delhi - 110058, IN
3 Drug Standardization Department, Dr D P Rastogi Central Research Institute for Homeopathy, Under Central Council for Research in Homeopathy, Noida - 201301, Uttar Pradesh, IN
Source
Journal of Natural Remedies, Vol 23, No 1 (2023), Pagination: 157-167Abstract
Background: Immunomodulation encompasses all therapeutic interventions aimed at modifying the immune response. The immune response augments are desirable to prevent infection in immunodeficiency states and fight established diseases. In this context, the present study investigated the effect of the homeopathic medicine, Camphora, in 6CH, 30CH, and 200CH potencies on immunomodulation in experimental animals. The acute oral toxicity study was also carried out in 6CH, 30CH, and 200CH potencies to determine the safe dose volume for the immunomodulatory study. Methodology: Acute oral toxicity studies of Camphora in 6CH, 30CH, and 200CH potencies were carried out as per OECD guideline 423 with slight modifications in Wistar albino rats. Humoral immunity, i.e., primary and secondary humoral responses, was assessed by measuring the hemagglutination titre of sheep red blood cells. Delayed-Type Hypersensitivity (DTH) was evaluated by measuring footpad thickness in BALB/c mice. Results: Camphora in 6CH, 30CH and 200CH potencies at a dose volume of 2000 µl/kg did not cause any mortality in the rats when administered as a single dose. Camphora in 6CH, 30CH and 200CH potencies showed augmented primary and secondary humoral responses against the SRBC antigen in BALB/c mice. However, the values were statistically non-significant except in the case of 6 CH potency (p<0.01), which showed statistically significant primary anti-SRBC antibodies. In the DTH assay, Camphora in 6CH, 30CH and 200CH potencies significantly decreased the paw volume ratio after 24 hrs of SRBC injection in the paw, thus insinuating its role in reducing cell-mediated immunity. Camphora in 6CH, 30CH and 200CH potencies also showed enhanced antibody titres and decreased paw volume compared to vehicle control, i.e. dispensing alcohol, suggesting that the effect was imminent because of Camphora. Conclusion: The study results indicate that Camphora in 6CH, 30CH, and 200 CH potencies is safe up to a dose volume of 2000 µl/kg when administered as a single dose, augments the primary and secondary humoral immunity, and decreases DTH in experimental animals. The current study’s findings suggest that Camphora might be useful as an immunomodulator in treating immune system disorders and infectious diseases and require further investigation to investigate its mechanism of action.Keywords
Camphora, Cell-mediated Immunity, Delayed Type Hypersensitivity, Homeopathic Medicine, Humoral Immunity, ImmunomodulationFull Text
References
- Kajaria D, Tripathi J, Tiwari S, Pandey B. Immunomodulatory effect of ethanolic extract of Shirishadi compound. AYU (An Int. Q. J. Res. Ayurveda). 2013; 34:322. https://doi. org/10.4103/0974-8520.123136 DOI: https://doi.org/10.4103/0974-8520.123136
- Abood WN, Fahmi I, Abdulla MA, Ismail S. Immunomodulatory effect of an isolated fraction from Tinospora crispa on intracellular expression of INF-γ, IL-6 and IL-8. BMC Complement. Altern. Med. 2014; 14:205. https://doi.org/10.1186/1472- 6882-14-205 DOI: https://doi.org/10.1186/1472-6882-14-205
- Yuandani, Jantan I, Rohani AS, Sumantri IB. Immunomodulatory Effects and Mechanisms of Curcuma Species and Their Bioactive Compounds: A Review. Front. Pharmacol. 2021; 12. https://doi. org/10.3389/fphar.2021.643119 DOI: https://doi.org/10.3389/fphar.2021.643119
- Acharya D, Shrivastava A. Indigenous herbal medicines. (Aavishkar Publishers, Distributors, 2008).
- Melchart D, et al. Results of Five Randomized Studies on the Immunomodulatory Activity of Preparations of Echinacea. J. Altern. Complement. Med. 1995; 1:145-160. https://doi.org/10.1089/acm.1995.1.145 DOI: https://doi.org/10.1089/acm.1995.1.145
- Bellavite P, Ortolani R, Conforti A. Immunology and Homeopathy. 3. Experimental Studies on Animal Models. Evidence-Based Complement. Altern. Med. 2006; 3:171-186. https://doi.org/10.1093/ecam/ nel016 DOI: https://doi.org/10.1093/ecam/nel016
- Kishore L, Singh R. Ameliorative effect of Cephalandra indica homeopathic preparation in STZ induced diabetic nephropathy rats. J. Ayurveda Integr. Med. 2019; 10:255-261. https://doi.org/10.1016/j. jaim.2017.07.010
- Sarkar A, Datta P, Das AK, Gomes A. Anti-rheumatoid and anti-oxidant activity of homeopathic Guaiacum officinale in an animal model. Homeopathy. 2014; 103:133-138. https://doi.org/10.1016/j. homp.2013.08.006 DOI: https://doi.org/10.1016/j.homp.2013.08.006
- Kishore L, Singh R. Ameliorative effect of Cephalandra indica homeopathic preparation in STZ induced diabetic nephropathy rats. J. Ayurveda Integr. Med. 2019; 10:255-261. https://doi.org/10.1016/j. jaim.2017.07.010 DOI: https://doi.org/10.1016/j.jaim.2017.07.010
- Lamba C, et al. Evaluation of homoeopathic treatment in polycystic ovary syndrome: A single blind, randomised, placebo-controlled pilot study. Indian J. Res. Homoeopath. 2018; 12:35. https://doi. org/10.4103/ijrh.ijrh_18_18 DOI: https://doi.org/10.4103/ijrh.ijrh_18_18
- Jyothilakshmi V, et al. Beneficial Antioxidative Effect of the Homeopathic Preparation of Berberis vulgaris in Alleviating Oxidative Stress in Experimental Urolithiasis. Res. Complement. Med. 2014; 21:7-12. https://doi.org/10.1159/000360240 DOI: https://doi.org/10.1159/000360240
- Hanif K, Kumar M, Singh N, Shukla R. Effect of homeopathic Lycopodium clavatum on memory functions and cerebral blood flow in memory impaired rats. Homeopathy. 2015; 104:24-28. https:// doi.org/10.1016/j.homp.2014.08.003 DOI: https://doi.org/10.1016/j.homp.2014.08.003
- Kundu T, Shaikh A, Jacob S. To evaluate the role of homoeopathic medicines as add-on therapy in patients with rheumatoid arthritis on NSAIDs: A retrospective study. Indian J. Res. Homoeopath. 2014; 8:24. https://doi.org/10.4103/0974-7168.129674 DOI: https://doi.org/10.4103/0974-7168.129674
- Kundu N, et al. Homeopathic Treatment of Rheumatoid Arthritis: An Open, Observational Trial. Homœopathic Links. 2019; 32:216-223. https://doi. org/10.1055/s-0039-3402080 DOI: https://doi.org/10.1055/s-0039-3402080
- Vo HW, Michalsen A, Brunjes R. Efficacy and tolerability of a complex homeopathic drug in children suffering from dry cough - A double-blind, placebo controlled, clinical trial. Drug Res. (Stuttg). 2018; 68:444-449. https://doi.org/10.1055/s-0043-124179 DOI: https://doi.org/10.1055/s-0043-124179
- Gibson R, Gibson S, MacNeill A, Buchanan W. Homoeopathic therapy in rheumatoid arthritis: evaluation by double‐blind clinical therapeutic trial. Br. J. Clin. Pharmacol. 1980; 9:453-459. https://doi. org/10.1111/j.1365-2125.1980.tb05840.x DOI: https://doi.org/10.1111/j.1365-2125.1980.tb05840.x
- Nahar K. Homoeopathy and immunomodulation: A narrative review article. Int. J. Homoeopath. Sci. 2021; 5:246-249. https://doi.org/10.33545/26164485.2021. v5.i2d.391 DOI: https://doi.org/10.33545/26164485.2021.v5.i2d.391
- Singh R, Jawaid T. Cinnamomum camphora (Kapur): Review. Pharmacogn. J. 2012; 4:1-5. https://doi. org/10.5530/pj.2012.28.1 DOI: https://doi.org/10.5530/pj.2012.28.1
- Ministry of Health and Family Welfare(India). Homoeopathic Pharmacopoeia of India. Govt. of India. 1990; 1:1-234.
- Ministry of AYUSH. Essential Drug List Homeopathy. 2013.
- Clarke JH. Materia Medica. 1902.
- Takacs M, et al. Adjunctive homeopathic treatment of hospitalised COVID-19 patients (COVIHOM): A retrospective case series. Complement. Ther. Clin. Pract. 2021; 44:101415. https://doi.org/10.1016/j. ctcp.2021.101415 DOI: https://doi.org/10.1016/j.ctcp.2021.101415
- Chen W, Vermaak I, Viljoen A. Camphor - A Fumigant during the Black Death and a Coveted Fragrant Wood in Ancient Egypt and Babylon - A Review. Molecules. 2013; 18:5434-5454. https://doi. org/10.3390/molecules18055434 DOI: https://doi.org/10.3390/molecules18055434
- Philpott NW. Intramuscular Injections of Camphor in the Treatment of Engorgement of the Breasts. Can. Med. Assoc. J. 1929; 20:494-5.
- Lee HJ, et al. In vitro anti-inflammatory and anti oxidative effects of Cinnamomum camphora extracts. J. Ethnopharmacol. 2006; 103:208-216. https://doi. org/10.1016/j.jep.2005.08.009 DOI: https://doi.org/10.1016/j.jep.2005.08.009
- Xiao S, et al. The anti-inflammatory potential of Cinnamomum camphora (L.) J. Presl essential oil in vitro and in vivo. J. Ethnopharmacol. 2021; 267:113516. https://doi.org/10.1016/j.jep.2020.113516 DOI: https://doi.org/10.1016/j.jep.2020.113516
- Bespalov A, Wicke K, Castagne V. Blinding and Randomization. Handb. Exp. Pharmacol. 2020; 257:81-100. https://doi.org/10.1007/164_2019_279 DOI: https://doi.org/10.1007/164_2019_279
- Al-Afifi NA, Alabsi AM, Bakri MM, Ramanathan A. Acute and sub-acute oral toxicity of Dracaena cinnabari resin methanol extract in rats. BMC Complement. Altern. Med. 2018; 18:50. https://doi. org/10.1186/s12906-018-2110-3 DOI: https://doi.org/10.1186/s12906-018-2110-3
- Puri A, et al. Immunostimulant Agents from Andrographis paniculata. J. Nat. Prod. 1993; 56:995- 999. https://doi.org/10.1021/np50097a002 DOI: https://doi.org/10.1021/np50097a002
- Singh S, et al. Role of homoeopathic mother tinctures in rheumatoid arthritis: An experimental study. Indian J. Res. Homoeopath. 2015; 9:42. https://doi. org/10.4103/0974-7168.154348 DOI: https://doi.org/10.4103/0974-7168.154348
- Singh S, et al. Attenuation of Complete Freund’s Adjuvant-induced arthritis by different dilutions of Eupatorium perfoliatum and Crotalus horridus and their safety evaluation. Indian J. Res. Homoeopath. 2019; 13:107-117. https://doi.org/10.4103/ijrh. ijrh_23_19 DOI: https://doi.org/10.4103/ijrh.ijrh_23_19
- Puri A, et al. Immunostimulant activity of Nyctanthes arbor-tristis L. J. Ethnopharmacol. 1994; 42:31-37. https://doi.org/10.1016/0378-8741(94)90020-5 DOI: https://doi.org/10.1016/0378-8741(94)90020-5
- Gokhale A, Damre A, Saraf M. Investigations into the immunomodulatory activity of Argyreia speciosa. J. Ethnopharmacol. 2003; 84:109-114. https://doi. org/10.1016/S0378-8741(02)00168-X DOI: https://doi.org/10.1016/S0378-8741(02)00168-X
- Percie du Sert N, et al. The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PLOS Biol. 2020; 18:e3000410. https://doi. org/10.1371/journal.pbio.3000410 DOI: https://doi.org/10.1371/journal.pbio.3000410
- Smith AJ, Clutton RE, Lilley E, Hansen KE. A. and Brattelid, T. PREPARE: guidelines for planning animal research and testing. Lab. Anim. 2018; 52:135- 141. https://doi.org/10.1177/0023677217724823 DOI: https://doi.org/10.1177/0023677217724823
- World Health Organization. Safety issues in the preparation of homeopathic medicines. World Health Organization. 2009.
- OECD. Test No. 423: Acute Oral toxicity - Acute Toxic Class Method. Oecd Guidel. Test. Chem. 2002; 1-14. https://doi.org/10.1787/9789264071001-en DOI: https://doi.org/10.1787/9789264071001-en
- Janhavi P, Divyashree S, Sanjailal KP, Muthukumar SP. DoseCal: a virtual calculator for dosage conversion between human and different animal species. Arch. Physiol. Biochem. 2022; 128(2):426-430. https://doi. org/10.1080/13813455.2019.1687523 DOI: https://doi.org/10.1080/13813455.2019.1687523
- Nfambi J, Bbosa GS, Sembajwe LF, Gakunga J, Kasolo JN. Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats. J. Basic Clin. Physiol. Pharmacol. 2015; 26. https://doi. org/10.1515/jbcpp-2014-0104 DOI: https://doi.org/10.1515/jbcpp-2014-0104
- Kind TJ, Osborn BA, Goldsby RA. Kuby Immunology. (W.H. Freeman and Company, 2007).
- Zaveri M, Gohil P, Jain S. Immunostimulant Activity of n-Butanol Fraction of Root Bark of Oroxylum indicum, vent. J. Immunotoxicol. 2006; 3:83-99. https://doi.org/10.1080/15476910600725942 DOI: https://doi.org/10.1080/15476910600725942
- Bhat J, Kabelitz D. An introduction to immunology and epigenetics. Epigenetics of the Immune System (Taylor and Francis, 2020). https://doi.org/10.1016/ B978-0-12-817964-2.09991-3
- Elgert KD. Immunology: understanding the immune system. Wiley. 2009.
- Burlingham WJ, Jankowska-Gan E, VanBuskirk AM, Pelletier RP, Orosz CG. Delayed Type Hypersensitivity Responses in Measuring Immunity. Elsevier. 2005; 407-418. https://doi.org/10.1016/B978-012455900- 4/50297-X DOI: https://doi.org/10.1016/B978-012455900-4/50297-X
- Safety Evaluation of Arsenicum Album in Acute and Sub-Acute Toxicity Studies in Rats
Abstract Views :150 |
PDF Views:0
Authors
Roshan Lal
1,
Mahima sharma
1,
Sangita Behera
1,
Raj Kumar Regar
1,
Deepika Tripathi
1,
G V Narasimha Kumar
2,
Satvinder Singh
1,
Digvijay Verma
3,
Pankaj Gupta
1,
Subhash Kaushik
3,
Anil Khurana
3
Affiliations
1 Department of Pharmacology, Dr. D. P. Rastogi, Central Council for Research in Homoeopathy, Noida - 201301, Uttar Pradesh, IN
2 Dr Anjali Chatterji Regional Research Institute for Homoeopathy, Kolkata - 700035, West Bengal, IN
3 Central Council for Research in Homoeopathy, New Delhi - 110023, IN
1 Department of Pharmacology, Dr. D. P. Rastogi, Central Council for Research in Homoeopathy, Noida - 201301, Uttar Pradesh, IN
2 Dr Anjali Chatterji Regional Research Institute for Homoeopathy, Kolkata - 700035, West Bengal, IN
3 Central Council for Research in Homoeopathy, New Delhi - 110023, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 30, No 2 (2023), Pagination: 233-247Abstract
Arsenic album is frequently prescribed in homoeopathy for many diseases. However, its safety data is not available. Thus, the study’s purpose is to evaluate the oral toxicity of Arsenic album 6C, 30C, and 200C in rats. Arsenic album (6C, 30C, and 200C) was given at 2000 μl/kg for acute toxicity and observed for up to 14 days. For subacute toxicity, it was given for 28 days and observed for clinical signs, change in body weight and Mortality. Hematological, biochemical, organ weight and histopathological analyses were assessed. Results indicate no mortality of arsenic album in acute toxicity and LD50 is >2000 μl/kg. In the subacute toxicity study, arsenic album (200 μl/kg) did not show any significant changes in above parameters. It may be concluded that the arsenic album (6C, 30C, and 200C) is safe and produces no toxicity when administered orally for a prolonged duration at 200 μl/kg in rats.Keywords
Complementary and Alternative Medicines, Homoeopathy, Safety, Toxicity.
References
- Briggs CJ, Norrie O. Regulation of Herbal and Homeopathic Medicines. Pharm Dev Regul. 2003; 1:245-259. https://doi. org/10.1159/000507952
- WHO. Safety issues in the preparation of homeopathic medicines. 2009. Retrieved from: https://apps.who.int/iris/ handle/10665/44238
- Singh S, Kumar R, Karwasra R, Kalra P, Rani S, Nayak D, et al. Evaluation of safety profile of homoeopathic mother tinctures. Indian J Res Homoeopathy. 2014; 8:81-86. https:// doi.org/10.4103/0974-7168.135640
- Habs M, Koller M. Material Risks of Homeopathic Medicinal Products: Regulatory Frameworks, Results of Preclinical Toxicology, and Clinical Meta-Analyses and Their Implications. Complement Med Res. 2021; 28:64-84. https://doi.org/10.1159/000507952
- Manchanda RK. Popularity, safety and quality of homoeopathic medicines. Indian J Res Homoeopathy. 2018; 12:191. https://doi.org/10.4103/ijrh.ijrh_72_18
- Sharma M, Prajapati S, Kumar A, Tripathi A, GVN Kumar, Gupta P. Effect of Acute Exposure of Belladonna Mother Tincture on Zebrafish Embryonic Development. Indian J Pharm Sci. 2021; 83:5. https://doi.org/10.36468/pharmaceutical- sciences.847
- Sharma M, Prajapati S, Kumar A, Tripathi A, GVN Kumar, Gupta P. Safety Evaluation of Syzygium jambolanum on the Development of Zebrafish Embryos. Indian J Pharm Educ Res. 2021; 55(1):198-204. https://doi.org/10.5530/ ijper.55.1.22
- Kirby BJ. Safety of homeopathic products. J R Soc Med. 2002; 95(5):221-222. https://doi.org/10.1258/jrsm.95.5.221
- Clarke JH. A dictionary of practical materia medica. Homoeopathic Publishing Company. 1902 10. Bellavite P, Signorini A, Marzotto M, Moratti E, Bonafini C, Olioso D. Cell sensitivity, non-linearity and inverse effects. Homeopathy. 2015; 104:139-160. https://doi.org/10.1016/j. homp.2015.02.002
- Kundu S, Mitra K, Bukhsh AK. Efficacy of a potentized homeopathic drug (Arsenicum-Album-30) in reducing cytotoxic effects produced by arsenic trioxide in mice: IV. Pathological changes, protein profiles, and content of DNA and RNA. Complement Ther Med. 2000; 8:157-165. https:// doi.org/10.1054/ctim.2000.0390
- Mallick P, Chakrabarti Mallick J, Guha B, Khuda-Bukhsh A. Ameliorating effect of microdoses of a potentized homeopathic drug, Arsenicum album, on arsenic-induced toxicity in mice. BMC Complement Altern Med. 2003; 3:1- 13. https://doi.org/10.1186/1472-6882-3-7
- Ive EC, Couchman I, Reddy L. Therapeutic effect of Arsenicum album on leukocytes. Int J Mol Sci. 2012; 13:3979-3987. https://doi.org/10.3390/ijms13033979
- Das D, De A, Dutta S, Biswas R, Boujedaini N, Khuda- Bukhsh AR. Potentized homeopathic drug Arsenicum Album 30C positively modulates protein biomarkers and gene expressions in Saccharomyces cerevisae exposed to arsenate. J Chin Integr Med. 2011; 9:752-760. https://doi. org/10.3736/jcim20110709
- Ernst E. The efficacy of herbal medicine–an overview. Fundam Clin Pharmacol. 2005; 19:405-409. https://doi. org/10.1111/j.1472-8206.2005.00335.x
- OECD Test No. 423. Acute oral toxicity-Acute toxic class method. 2002. Retrieved from: https://www.oecd-ilibrary. org/environment/test-no-423-acute-oral-toxicity-acutetoxic- class-method_9789264071001-en
- OECD Test No. 407. Repeated Dose 28-day Oral Toxicity Study in Rodents. 2008. https://doi.org/10.1787/ 9789264070684-en
- Olfert E, Bhasin J, Latt R, McCutcheon K, Rainnie D, Schunk M. CCAC Guidelines on: Choosing an Appropriate Endpoint in Experiments Using Animals for Research, Teaching and Testing. 1998. Retrieved from: https://ccac. ca/Documents/Standards/Guidelines/Appropriate_endpoint. pdf
- Chitra B, Ramaswamy R, Suba V. Toxicity evaluation of Purn. a Cantirotaya Centuram, a Siddha medicine in Wistar rats. Int Sch Res Notices. 2015; 473296. https://doi. org/10.1155/2015/473296
- Wren AF, Cleary M, Frantz C, Melton S, Norris L. 90-day oral toxicity study of a grape seed extract (IH636) in rats. J Agric Food Chem. 2002; 50:2180-2192. https://doi. org/10.1021/jf011066w
- Rao JV. Toxic effects of novel organophosphorus insecticide (RPR-V) on.certain.biochemical parameters of euryhaline fish, Oreochromis mossambicus. Pestic Biochem Phys. 2006; 86:78-84. https://doi.org/10.1016/j.pestbp.2006.01.008
- Mukinda JT, Syce JA. Acute and chronic toxicity of the aqueous extract of Artemisia afra in rodents. J Ethnopharmacol. 2007; 112:138-144. https://doi. org/10.1016/j.jep.2007.02.011
- Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, et al. Concordance of the toxicity of pharmaceuticals in humans and in animals. Regul Toxicol Pharmacol. 2000; 32:56-67. https://doi.org/10.1006/rtph.2000.1399
- Olorunnisola O, Bradley G, Afolayan A. Acute and subchronic toxicity studies of methanolic extract of Tulbaghia violacea rhizomes in Wistar rats. Afr J Biotechnol. 2012, 11:14934-4940
- Anderson N, Borlak J. Molecular mechanisms and therapeutic targets in steatosis and steatohepatitis. Pharmacol Rev. 2008; 60:311-357. https://doi.org/10.1124/pr.108.00001
- Schalm OW. Schalm’s veterinary hematology; 2000
- Loge JP, Lange RD, Moore CV. Characterization of the anemia associated with chronic renal insufficiency. Am J Med.1958; 24:4-18. https://doi.org/10.1016/0002-9343(58)90357-7
- Wallig MA, Bolon B, Haschek WM, Rousseaux CG. Fundamentals of toxicologic pathology. Academic press; 2017
- Nigatu TA, Afework M, Urga K, Ergete W, Makonnen E. Toxicological investigation of acute and chronic treatment with Gnidia stenophylla Gilg root extract on some blood parameters and histopathology of spleen, liver and kidney in mice. BMC research notes. 2017; 10:1-13. https://doi. org/10.1186/s13104-017-2964-3
- Michael B, Yano B, Sellers RS, Perry R, Morton D, Roome N, et al. Evaluation of organ weights for rodent and nonrodent toxicity studies: a review of regulatory guidelines and a survey of current practices. Toxicol Pathol. 2007; 35:742-750. https://doi.org/10.1080/01926230701595292